FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9161407
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1721831.8794383875751837No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC570130.6223170742223328Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC97500.047.70007715
GAGCACA95800.047.4856416
GCACACG50300.046.75121718
ACACGAG33050.042.99188620
AGCACAC63850.041.92638417
GATCGAG30600.040.8408628
CACACGA36100.040.03778519
AAGAGCA179900.038.69137614
AGATAGA95850.037.0701567
CACGAGA39650.036.18945721
GATAGAT100250.035.5477038
GAGCAAG60700.032.68728616
CACGTAG26250.031.46473921
ATAGATC113550.031.4140079
AGAGAGA97250.031.30605915
GCACAAG55250.031.2886118
CAGATAG44050.030.0404136
AGATCGA43600.029.3863127
AAAGGGG93700.029.13518366
CGGGGAC11350.029.0238341
Produced by FastQC (version 0.11.5)