FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6939003
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG968291.3954310150896316No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC276710.3987748672251619Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC52800.041.95467415
GAGCACA51000.041.78738416
GCACACG25450.041.11380818
AGCACAC35400.034.00557317
ACACGAG20900.033.65801620
CACACGA22100.032.30388319
AAGAGCA107400.031.67292814
CACGAGA24550.028.51152221
CGGGGAC8550.025.8247871
CAGATCG82750.025.2556086
GATCGAG15800.025.0360958
AGAGATC63100.024.51251417
AAAGGGG54700.024.50615966
GATAGAT59450.024.142228
AGATAGA60150.024.0944047
GCACAAG35650.023.95160518
AAGGGGG55950.023.52093967
ATAGATC62850.023.2814459
CGGGGAT13650.023.1085221
GAGCAAG44100.023.09151616
Produced by FastQC (version 0.11.5)