FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939267
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1876671.8881372238013123No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC703540.7078389181013046Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC133250.051.08089415
GAGCACA132450.050.3051416
GCACACG60400.048.14759418
ACACGAG44350.045.45285820
CACACGA44600.045.43325419
AAGAGCA237650.043.4857614
AGCACAC81350.043.14602317
GATCGAG37450.041.3170788
CACGAGA49700.040.98254821
GCACAAG74250.039.82637418
AGATAGA118400.038.43777
GATAGAT117800.038.3357168
GAGCAAG81550.037.93374316
AGAGAGA125200.036.0571615
GCAGATC93500.033.7745
ATAGATC133500.033.7476359
AGCACAA88950.033.4403117
GAGAGAT135350.033.145716
AGATCGA48800.032.999227
AGAGATC139600.031.8859317
Produced by FastQC (version 0.11.5)