FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9396038
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1736521.8481406737605788No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC246120.26194019223847326Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25650.036.9740518
AGAGCAC59700.035.05416515
GAGCACA58200.034.33411416
AGCACAC39650.027.18443117
CGGGGAC13000.026.6893831
ACACGAG22350.025.52356320
CACACGA23950.024.25643219
CAGATCG85850.024.2220846
AAGAGCA127750.024.13468414
CGGGGAG14550.024.0870551
CGGGGTT14800.021.7857651
CGGGGAT19850.021.3634591
AAAGGGG63350.020.82796366
GCACAAG39300.020.5700118
CACGAGA28850.020.25835621
AGAGATC67350.020.10879717
AAGGGGG64600.019.99173567
GATCGAG19700.019.9023028
ATAGATC73400.019.8396919
CGCGGAT7500.019.6261351
Produced by FastQC (version 0.11.5)