FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP04-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP04-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8356951
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1703352.0382433736897583No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC753620.9017882239587142Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA122300.050.5873816
AGAGCAC127050.050.51512515
GCACACG57350.048.32743518
ACACGAG39900.046.92746420
CACACGA40850.046.17744419
AAGAGCA217050.045.54872514
AGCACAC74150.044.4558317
CACGAGA44000.042.5549321
GCACAAG64500.041.3425518
AGATAGA113850.041.232857
GATCGAG33300.040.682648
GATAGAT115600.040.4264688
GAGCAAG76050.040.16996416
AGAGAGA121850.037.535915
ATAGATC129450.036.1271559
GCAGATC87550.035.828455
GAGAGAT129600.034.9124216
AGCACAA77800.034.36392217
CGGGGAC9550.034.1333471
AGAGATC132800.034.01865417
Produced by FastQC (version 0.11.5)