FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP04-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP04-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8265803
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1388541.679860988702489No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC334270.4044011211009989Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC66400.043.1115715
GAGCACA64250.042.37423716
GCACACG30950.039.79972518
ACACGAG23900.036.0210420
AGCACAC39750.034.50974317
CACACGA25350.033.82159419
AAGAGCA134050.033.78209314
GATCGAG22700.030.3792978
CACGAGA29900.029.26091421
GCACAAG43800.029.16196618
AGATAGA76850.028.1051927
GATAGAT76700.028.0227268
CAGATCG119200.027.3708026
AGAGAGA83950.026.97069715
GAGCAAG53650.026.8734216
CGGGGAC10350.026.7480621
GCAGATC56350.026.5895425
AGAGATC84750.026.3435217
GAGAGAT87550.025.62107316
ATAGATC84550.025.4617549
Produced by FastQC (version 0.11.5)