FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP03-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP03-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056114
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480592.098307935501042No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC735301.0420750004889376Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC104850.054.20446415
GAGCACA100600.053.04784816
GCACACG49200.048.79236618
AGATAGA127950.048.5369457
AAGAGCA194200.048.36889314
GATCGAG42000.048.1764268
CACACGA33450.047.9160119
ACACGAG33950.047.2126720
GATAGAT132450.046.4911088
AGCACAC60450.045.7326917
GAGCAAG76700.043.79976716
CAGATAG55750.042.4492656
GCACAAG57050.041.52655418
AGATCGA49850.041.5031857
AGAGAGA124150.041.38060415
ATAGATC148800.041.2644969
ACACTCT39100.040.332391
GCAGATC82900.039.740025
CACGAGA41700.039.44552621
AGAGCAA87200.039.12961615
Produced by FastQC (version 0.11.5)