FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP03-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP03-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794291
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1411721.605268690790423No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC313720.3567314295148978Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA86200.045.590716
AGAGCAC89800.045.1656615
GCACACG39250.041.99449518
ACACGAG31500.038.55225820
AAGAGCA174750.037.9496814
CACACGA33600.036.24628419
AGCACAC53150.036.2126817
CACGAGA35050.034.74738721
GAGCAAG68550.033.48884216
GCACAAG55750.033.457518
AGAGAGA107050.032.59190815
AGAGATC108850.031.86005217
CAGATCG142550.031.7023336
GAGAGAT110450.031.46206716
GATAGAT84700.028.8880738
GCAGATC67250.028.681965
AGATAGA85850.028.3383317
GATCGAG24150.028.2645638
AGAGCAA86050.027.69475415
AGCACAA69000.027.48852517
Produced by FastQC (version 0.11.5)