FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP02-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP02-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9871020
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6393266.476797737214595Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1387331.4054575920218983No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT283220.286920703230264Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC243620.24680326855785928Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC210460.213209982352381Illumina Single End PCR Primer 1 (98% over 50bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC153440.1554449286902468Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC145320.1472188284493396Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC115220.11672552583218351Illumina Single End PCR Primer 1 (100% over 38bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG103350.10470042609578342Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGA552150.062.634367
GATCGAG168450.062.4496778
ACACTCT143300.061.994521
CAGATAG214700.060.2484326
GATAGAT582100.059.3575978
AGAGCAC371650.059.33780715
AGATCGA183850.059.2179647
GAGCACA363250.058.8594516
CACACGA112700.057.6955119
CACTCTT153150.057.498152
ACACGAG113500.057.3825120
ATCGAGA183600.057.2572639
AAGAGCA607200.056.79698614
GCACACG174650.056.20168318
GTGACTG67050.055.925071
GAGCAAG203800.055.58007416
TAGATAG169650.055.3024336
AGCACAC208250.055.2325817
GCACAAG165200.055.0742218
AGATCAG153450.053.7255367
Produced by FastQC (version 0.11.5)