FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP02-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP02-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084089
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG970011.369279804361577No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC294510.4157344719977403Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG31650.046.10730718
GAGCACA65750.045.82597716
AGAGCAC67800.045.52397515
AGCACAC41650.041.00267817
ACACGAG22250.040.1093120
CACACGA23700.037.80249819
AAGAGCA128050.036.62198314
CACGAGA26750.033.75444421
CAGATCG103650.031.3766946
CGGGGAC9250.030.3071331
GCACAAG39750.030.28507818
GAGCAAG47650.029.59691616
AGATAGA64100.029.381427
GATAGAT64750.028.8700088
GCAGATC50350.028.021075
AGAGATC66750.027.36696617
ATAGATC70450.026.632439
GATCGAG18800.026.2546798
CACGTAG19300.025.9306721
AGAGAGA70650.025.80636415
Produced by FastQC (version 0.11.5)