FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP01-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP01-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9510886
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2713432.8529728986342597Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1683301.769866655956133No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT122650.12895749144716906Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC103880.10922221126401895Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC338900.059.6438915
GAGCACA331450.059.611316
CACACGA105400.058.03996319
ACACGAG107550.056.9459820
AAGAGCA531750.056.4472514
GCACACG165350.056.27586418
AGCACAC196400.055.34367817
GATCGAG68750.054.7879038
AGATAGA252100.054.3636477
GCACAAG150350.054.18605818
GAGCAAG167700.053.90103516
CACGAGA113650.053.89005321
GATAGAT264800.051.5046048
AGCACAA159150.051.2338917
ACACTCT76550.050.0769461
CAGATAG97550.050.0255666
AGAGAGA269800.049.1724915
CACGTAG60800.048.92776521
AGAGCAA188650.048.89888415
ACACGTA61250.046.96790320
Produced by FastQC (version 0.11.5)