FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP01-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP01-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172470
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1702011.6731531280013607No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC771860.7587734345739039Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA105800.047.00215516
AGAGCAC109950.046.15110815
GCACACG47350.044.64087718
GATCGAG38650.040.394168
CACACGA35250.040.10963419
AAGAGCA203550.039.13063414
ACACGAG36150.039.01539220
AGCACAC64650.038.27015317
AGATAGA130250.036.6047067
GATAGAT130350.036.1197288
CACGAGA39950.035.8300921
GCACAAG66400.035.3120118
GAGCAAG76750.034.1973316
ATAGATC138700.034.0202989
AAGGGGG128850.032.8399567
AAAGGGG130400.032.52995766
AGATCGA52450.031.5684417
GCAGATC77850.031.0729565
CAGATCG188300.030.953216
CAGATAG57950.030.8076446
Produced by FastQC (version 0.11.5)