FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP00-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP00-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473573
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5549965.299013049319464Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1747551.6685327920089925No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT236920.22620742701654917Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC194390.18560046318481763Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC175430.1674977584058468Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC144010.13749844489554805Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC125710.12002589756141481Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA596550.062.50858716
AGAGCAC610300.062.49993515
CACACGA190300.061.79110319
AAGAGCA920750.061.16516514
ACACGAG193600.060.9568120
GAGCAAG274150.060.2867716
GCACACG287350.059.72276318
AGCACAC337850.059.4426817
GCACAAG265950.059.37036518
CACGAGA200000.059.2514621
AGATAGA382800.058.6186837
AGCACAA270950.058.19532817
ACACTCT122400.058.03021
AGAGCAA295950.057.2772615
GATCGAG98650.056.918188
AGAGAGA478300.056.4824315
CAGATAG142900.055.6828276
CACTCTT127550.055.1939852
GTGACTG74950.054.9348531
GATAGAT407200.054.9076548
Produced by FastQC (version 0.11.5)