FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n02_CIP00-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n02_CIP00-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704733
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2304832.647789426740602Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1299871.4932910636087287No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT111810.12844736306099222Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC97200.11166339047963907Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG74200.056.6158648
AGATAGA253400.054.9853827
AGAGCAC181600.054.34121315
GAGCACA175150.054.08466716
GATAGAT257400.053.9800578
GCACACG84850.052.41972718
CACACGA56750.051.7391919
CAGATAG100200.050.3817566
ACACTCT64400.050.0650751
AGCACAC103000.049.9774417
AGATCGA86650.049.6539847
AAGAGCA301700.049.4598614
ACACGAG60400.048.72928620
ATAGATC289450.048.0263569
ATCGAGA87750.047.9923259
CACGAGA63000.046.4415521
GCACAAG88350.046.3425918
GAGCAAG101200.045.67918816
CACTCTT71100.044.978942
GTGACTG43700.043.7868961
Produced by FastQC (version 0.11.5)