FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10393544
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1024830.9860255558642943TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG670290.6449099556416945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT1787850.048.3573651
CGCGTAT210200.040.45030669
GTTCAGA210350.039.280021
TCGCGTA217850.037.2298568
GCGTATG277750.037.2165770
CGGGGAT27300.037.18453669
TCGGGGA29200.037.16222468
ACGTGTG2349000.037.0353552
GGGTGGC31400.036.4539970
ATCGGGT50600.035.90390867
GGGGATG31700.035.33602570
ATCGCGT256950.035.25660767
TCAATCG84900.034.8363164
CCTCGCG15550.034.21653766
TCGCGGA54550.034.1381368
ATGCCGG72350.033.6258570
GGATGGC24050.033.4764870
GCGGATG73350.032.40385470
GTGACTG19900.032.1958771
GTAATCG51650.032.12115564
Produced by FastQC (version 0.11.5)