FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9161407
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG101110.11036514369463118TruSeq Adapter, Index 8 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG41800.030.2397171
CGTGTGC76900.025.2704771
GTGTGCT86250.022.5274432
AAAAAGG149650.020.25507570
GCGTGGA22100.019.63912270
TGTGCTC115250.018.441431
GTGCTCT117500.018.1152252
GACGTGT27100.018.0886251
GGGTGGA28950.016.8057970
GCGTGAA29500.016.49075360
TGCGTGG25550.016.02830769
TGCCGTC62350.015.77311649
CTTCCGA224800.015.5574263
CGGGGTG46150.015.54801170
CCGATCT218900.015.5446866
TCCGATC221750.015.5028545
GGCGGGG39450.015.43813870
CTGCTTG67200.015.36386258
TCTTCCG231900.015.1871152
AAAAAAG221850.014.84645969
Produced by FastQC (version 0.11.5)