FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6939003
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG309940.44666359129690536TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC31400.028.882181
ATGCCGG14550.023.81585170
ACGTGTG21900.022.2244241
GTGTGCT41250.021.8963152
GCTTGAA128100.019.88974860
CTGCTTG126600.019.5170858
TGCTTGA128750.019.46299259
CTTGAAA140650.018.46364461
TCCGATC103350.016.5978375
GTGCTCT57600.016.5318622
TGTGCTC57650.016.2777841
TCTGCTT156200.016.22188857
CCGATCT105650.016.1370946
CTTCTGC159700.015.80085155
CTTCCGA110750.015.5205393
CCGTCTT167950.015.39978151
GCCGTCT171250.015.04171550
TTCTGCT168050.014.89057256
GTCTTCT174100.014.81558753
TGAAAAA188550.014.71983763
Produced by FastQC (version 0.11.5)