FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP05-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP05-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939267
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG817580.8225757492982128TruSeq Adapter, Index 9 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC73850.033.755411
ATGCCGG30500.031.10066470
ACGTGTG42050.028.5589351
GTGTGCT93500.026.956042
CTTGAAA276000.023.30746861
TGTGCTC144750.022.252721
GTGCTCT148100.021.9581552
GCTTGAA293900.021.68504560
GCCGTCG31000.020.5499170
TGAAAAA327200.020.45219263
TGCTTGA310600.020.27110959
TTGAAAA332000.019.68200362
GAAAAAA345700.019.62142664
AAAAGGG89150.019.16014170
CTGCTTG331500.018.81350758
AAAAAGG185150.017.16573370
CTTCCGA302950.016.9045723
TCTGCTT376500.016.74149957
TCCGATC302500.016.6635675
TCTTCCG310950.016.5936572
Produced by FastQC (version 0.11.5)