FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP05-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP05-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9396038
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG121140.12892668165028706TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG30750.021.6355921
CGTGTGC42850.020.5925461
GTGTGCT54150.016.4207822
GCCGTCT60350.014.38201550
CGGGGGG7150.014.19672470
TCCGATC109100.014.1181335
CCGATCT107050.014.0941856
TGCCGTC73400.014.01834149
GACGTGT19850.013.9356391
CTGCTTG68650.013.61181658
ATGCCGT75900.013.41827148
GTGCTCT69450.013.4081232
TGTGCTC69000.013.3972341
TATGCCG83150.013.30056847
CTTCCGA117550.013.1331813
GCTTGAA88150.012.78442560
TGCTTGA79450.012.68656259
GCCGGGG11500.012.4790768
CCGTCTT76650.012.37378351
GTATGCC95250.012.34581146
Produced by FastQC (version 0.11.5)