FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP04-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP04-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8356951
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG190670.22815737462143787TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG88290.10564857924858001TruSeq Adapter, Index 10 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG42900.032.401681
CGTGTGC94150.028.9701961
GTGTGCT99700.027.5283282
GCGTGGA26700.026.6130370
AAAAAGG164950.025.10394570
GCGTGAA42300.022.4223660
TGTGCTC142250.021.3649441
TGCGTGA37250.021.32796159
GTGCTCT146250.020.8488082
TGCGTGG29200.020.37853869
CTGCTTG87250.020.17668258
GCTTGAA100500.020.12862860
TGCTTGA90400.019.7834659
GGCGGGG46150.019.49265570
GGGTGGA25800.019.12973470
CGTGAAA53850.018.39324261
CGGGGTG51100.018.08392170
AAAAAAG248400.018.0088669
CGTCTGC46500.017.98838255
TGCCGTC109200.017.72350749
Produced by FastQC (version 0.11.5)