FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP04-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP04-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8265803
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG174400.21098978526345233TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC51850.027.8875271
ACGTGTG30600.024.3706381
GTGTGCT61400.023.6031342
TGTGCTC81250.017.5810781
GTGCTCT82350.017.4709282
TCCGATC156050.016.0390055
CCGATCT155150.015.996676
CTTCCGA160100.015.9393773
TCTTCCG169500.015.1587752
CGGGGGG7450.015.03451470
TTCCGAT170950.014.8458144
CTGCTTG77100.014.52584458
GGCGGGG14850.014.37801970
GCTTGAA90000.014.23277760
AAAAAGG127950.014.11578870
GCGTGGA15750.014.00089270
GACGTGT19600.013.9330841
TGCTTGA85450.013.88460159
CTCTTCC188750.013.8561421
CGATCTG69450.013.50583657
Produced by FastQC (version 0.11.5)