FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP03-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP03-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056114
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1275001.8069435952990556TruSeq Adapter, Index 11 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC84850.033.258031
ATGCCGG50000.033.18263270
ACGTGTG42150.032.644371
AAAAGGG78800.031.22709870
CTTGAAA320800.030.09022961
GTGTGCT97300.029.1412732
TGAAAAA358350.028.87125863
TTGAAAA345050.028.36094562
AAAAAGG193150.028.0167270
GAAAAAA374900.027.82098864
GCTTGAA368950.025.73567460
TGCTTGA387900.023.7114959
TGTGCTC144950.023.502171
GTGCTCT150050.022.8159852
GACGTGT21050.022.6203941
CTGCTTG413750.022.12823958
AAAAAAG270850.020.89713769
TCTGCTT451650.020.65105857
TTCTGCT453250.019.91395856
CTTCTGC473750.019.59919555
Produced by FastQC (version 0.11.5)