FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP03-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP03-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8794291
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG337960.3842947657747509TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC56500.029.8047921
ATGCCGG33050.028.382470
GTGTGCT66650.025.5244542
ACGTGTG33000.023.5520381
TGTGCTC98300.021.2979181
GTGCTCT102450.020.6027742
GCCGTCG36600.020.1784270
TCCGATC209650.017.9986255
CCGATCT208900.017.9291936
GACGTGT22200.017.5048941
CTTCCGA218850.017.4500263
TCTTCCG226250.017.095982
TTCCGAT228750.016.6335094
CTCTTCC247950.015.6869831
GCTTGAA158750.015.58679660
CGATCTG87850.015.3778897
TGCTTGA164050.014.82713659
CTGCTTG163150.014.69441258
CGATCTA78450.014.5437327
GCTCTTC181450.014.507122
Produced by FastQC (version 0.11.5)