FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP02-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP02-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9871020
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4714834.776436477689236TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG844410.8554435103971019TruSeq Adapter, Index 12 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT347900.3524458465285249TruSeq Adapter, Index 12 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA292710.2965347046201912TruSeq Adapter, Index 12 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATT263000.2664364979505664TruSeq Adapter, Index 12 (97% over 48bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG192220.1947316488063037TruSeq Adapter, Index 12 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA172550.17480463011927846TruSeq Adapter, Index 12 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG171800.17404483022017989TruSeq Adapter, Index 12 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155720.15775472038350646No Hit
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG132760.1344947128057688TruSeq Adapter, Index 12 (100% over 37bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA131230.13294472101160773TruSeq Adapter, Index 12 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATT123860.12547842066979906TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG122140.12373594623453302TruSeq Adapter, Index 12 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG207500.053.40688370
AAAAAGG703300.045.7631170
TGAAAAA1019150.044.20192763
TTGAAAA873250.043.57372362
GAAAAAA1082900.042.39916664
ACGTGTG114900.041.564231
CTTGAAA929800.039.99384761
GTAGGGA30250.038.9993482
AAAAAAG859300.038.95047469
TGTAGGG30700.038.6622161
GACGTGT48100.036.4685131
GCGTGGA78200.034.2867670
CGTGAAA172950.031.46760261
GTGAAAA188900.031.3674262
GTGTGCT289500.031.318722
GCTTGAA1162250.031.13654160
GCTTGGA62100.030.77552470
CGTGTGC297500.030.4345571
AGGGGGG18150.030.2864571
GGGTGGA49200.030.2362770
Produced by FastQC (version 0.11.5)