FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP02-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP02-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084089
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG90450.1276804963912791TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG83340.11764392005803427TruSeq Adapter, Index 5 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG27350.027.9070931
CGTGTGC48450.024.2084221
GTGTGCT53950.021.7361662
GACGTGT16250.018.7448221
TGTGCTC74650.018.1508331
GTGCTCT77350.017.7853262
GCCGGGG12850.017.70541470
CTGCTTG74650.017.4405458
GCTTGAA83850.017.15503960
TGCTTGA80650.016.66390259
GGCGGGG9750.015.79588470
GCCGTCT82100.015.38912550
GCGTGGA13200.015.37977370
TGCCGGG16500.014.63750769
TCCGATC139050.014.5506835
CCGATCT139050.014.3995366
AAAAAGG115600.014.35212970
CCGTCTT97100.014.165215551
CTTCCGA146200.014.0306163
TGCCGTC102750.013.99941449
Produced by FastQC (version 0.11.5)