FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP01-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP01-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9510886
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG2785842.9291067099321766TruSeq Adapter, Index 13 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA150690.1584394976451195TruSeq Adapter, Index 13 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA123810.13017714648246229TruSeq Adapter, Index 13 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG100750.043.715161
AAAAGGG139100.037.62141870
AAAAAGG372200.035.26760570
CGTGTGC224200.035.150891
TGAAAAA657600.033.83976463
TTGAAAA617450.032.93301462
CTTGAAA615750.032.63157361
GTGTGCT242450.032.5577662
GAAAAAA693800.032.35730764
ATGCCGG132450.031.92537770
AAAAAAG481100.028.82022969
GACGTGT38600.028.1171191
GCTTGAA726250.027.11696460
CGTCTTG76400.026.71137670
TGCTTGA753850.025.22772859
TGTGCTC373100.024.2942351
GTGCTCT387100.023.5295892
CGTCGTG41700.023.5040770
CTGCTTG808500.023.34066858
TCTGCTT871200.022.19513957
Produced by FastQC (version 0.11.5)