FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP01-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP01-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10172470
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG867140.8524380017832444TruSeq Adapter, Index 6 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC65700.028.5098821
ACGTGTG36150.026.5368421
ATGCCGG47250.026.44679370
GTGTGCT77350.024.166352
CTTGAAA264300.023.45183261
GCTTGAA265500.022.80502760
TGTGCTC114850.022.8022421
GTGCTCT119200.022.024962
TGCTTGA274300.021.4479959
CTGCTTG284650.020.40983658
TGAAAAA346450.019.95208763
AAAAAGG199400.019.11654370
TTGAAAA339750.018.63531162
AAAAGGG91400.018.49726770
GAAAAAA379550.018.42436464
TCTGCTT333400.017.96094357
GCCGTCG53500.017.53426770
CTTCTGC354800.017.15381455
TTCTGCT342750.016.85832256
CTTCCGA254350.016.7216423
Produced by FastQC (version 0.11.5)