FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP00-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP00-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473573
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG5875885.610196252988355TruSeq Adapter, Index 15 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTC291810.27861552117887567TruSeq Adapter, Index 15 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA229920.2195239389652414TruSeq Adapter, Index 15 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATATCGTATG210130.20062876346018688TruSeq Adapter, Index 15 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA190440.18182906635586538TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA169870.16218915932509373TruSeq Adapter, Index 15 (100% over 39bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT158570.15140010004226828TruSeq Adapter, Index 15 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125350.11968217531877613No Hit
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA123790.11819271226734182TruSeq Adapter, Index 15 (97% over 39bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT114670.10948508212049507TruSeq Adapter, Index 15 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG231950.048.6820170
ACGTGTG155900.046.0351141
TGAAAAA1156200.039.9345963
TTGAAAA1080650.039.66428462
AAAAAGG637900.039.64457770
GAAAAAA1192950.039.04269864
CTTGAAA1117150.038.0423661
CGTGTGC390550.037.9090161
GACGTGT53500.036.9722751
CGTCTTG116200.036.41821770
ATGCCGG221800.036.09135470
GTGTGCT420200.035.254042
AAAAAAG783400.033.5509969
GAATCGG22950.031.2673032
GCTTGAA1347650.031.08117160
GGGAAAG69250.029.2206271
TGCTTGA1402000.028.97967359
CGTCGTG61900.028.6126770
AAAGGGG40500.028.34766870
TGTAGGG17900.027.9681971
Produced by FastQC (version 0.11.5)