FastQCFastQC Report
Thu 14 Mar 2019
HGWHWAFXY_n01_CIP00-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHWAFXY_n01_CIP00-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8704733
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG844060.969656392677409TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG239370.27498833106081483TruSeq Adapter, Index 7 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT138070.158614859295512TruSeq Adapter, Index 7 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTG118670.13632813321212722TruSeq Adapter, Index 7 (97% over 49bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC116220.13351357244386475TruSeq Adapter, Index 7 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATT91300.10488546862953753TruSeq Adapter, Index 7 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATG89910.10328863619366614TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATG87300.10029026737523139TruSeq Adapter, Index 7 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG312700.038.0469370
ACGTGTG67800.037.9562841
GCGTGGA48150.033.22126870
GACGTGT30800.031.375051
GGGTGGA47250.031.33537370
GCTTGAA222950.030.37525460
CTTGAAA236050.029.87661
AAAAGGG96300.029.40482170
AAAAAAG435600.028.99211969
CGTGAAA91300.028.9802761
TGCTTGA212250.028.88901959
TGAAAAA382850.028.85158763
GTGTGCT155350.027.9874942
GCGTGAA93700.027.19181660
CGTGTAA31150.026.9652661
CTGCTTG225550.026.82863458
CGTGTGC163350.026.6212561
TGCGTGA80050.026.10113759
TGCGTGG46600.025.98911369
GAAAAAA456200.025.40184864
Produced by FastQC (version 0.11.5)