FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_hto.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_hto.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24219733
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4021981.6606211141964282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACA8288550.043.8814161
TCACATC8273850.043.80843
ATCACAT8309050.043.8018722
ATCGGTC8280350.043.7167247
CATCGGT8295700.043.7092066
TCGGTCC8252700.043.6957748
ACATCGG8317550.043.6952445
CGGTCCC8242900.043.665439
CATGTTA2340150.043.6532822
CACATCG8291350.043.6512874
GGTCCCA9659100.043.58618510
CGTATTC2178900.043.564617
CTAACGG2203300.043.5394066
CCGTCCC2312050.043.5154769
AGCTTAC2485800.043.514781
GTATTCC2181450.043.5146948
TATCCCC2449500.043.508728
GTCCCAT12020800.043.48030511
ACTGTCT2215050.043.4790081
TGTCTAA2214900.043.4655273
Produced by FastQC (version 0.11.2)