FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_cdna_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_cdna_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78764942
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55759837.079270114869125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5745390.7294349305811715No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3527590.4478629591322495No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2267030.28782221410129394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6456500.042.3399541
AGCAGTG6762250.040.35292
TCAACGC7081500.038.50023712
ATCAACG7086950.038.49818811
TGGTATC7081000.038.4551857
GTGGTAT7092050.038.415056
CAACGCA7106450.038.39718613
TATCAAC7137650.038.26735710
GTATCAA7121500.038.258719
GGTATCA7131650.038.2261858
AGTGGTA7141450.038.18335
AACGCAG7180700.038.03128414
CAGTGGT7173150.037.976314
ACGCAGA7193900.037.90184415
AGAGTAC7145650.037.76024219
CGCAGAG7231500.037.72969416
GCAGAGT7235700.037.50854517
CAGAGTA7235650.037.46242518
GAGTACT7159050.037.4018620
GCAGTGG7312800.037.295893