FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_cdna_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_cdna_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75449369
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53863207.138986145795334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4651530.6165101261483048No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3385420.448700902985683No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2177590.2886160651655019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6216050.042.400911
AGCAGTG6518650.040.358482
TCAACGC6829250.038.50450512
TGGTATC6810750.038.5005767
ATCAACG6835650.038.49852411
GTGGTAT6823250.038.4628566
CAACGCA6849650.038.421413
GTATCAA6864800.038.2832579
TATCAAC6884450.038.26731510
GGTATCA6865550.038.2512448
AGTGGTA6870150.038.222185
AACGCAG6920650.038.05883814
CAGTGGT6906000.037.9971584
ACGCAGA6938150.037.9160815
AGAGTAC6884100.037.77727519
CGCAGAG6962550.037.7730716
GCAGAGT6968000.037.5326217
CAGAGTA6973600.037.44898618
GAGTACT6904300.037.4031420
GTACTTT6883050.037.30436322