FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_cdna_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_cdna_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124340384
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT86902356.9890688129127865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8079860.6498178419651656No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5598100.4502237985689348No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3577040.28768127336650334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT10030450.042.372241
AGCAGTG10522600.040.3100242
TCAACGC11054300.038.3679712
ATCAACG11064400.038.3623811
TGGTATC11035700.038.3540347
GTGGTAT11053800.038.3084266
CAACGCA11097000.038.25675213
GTATCAA11111200.038.1523369
TATCAAC11141650.038.13769510
AGTGGTA11123400.038.0989845
GGTATCA11123950.038.0978368
CAGTGGT11170800.037.898784
AACGCAG11212800.037.88255714
ACGCAGA11240000.037.74455315
AGAGTAC11149500.037.63821819
CGCAGAG11288100.037.5918816
GCAGAGT11297250.037.36001217
CAGAGTA11296000.037.30726218
GAGTACT11181500.037.25146520
GTACTTT11138850.037.19059422