FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_cdna_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_cdna_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76202122
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54624207.168330561713229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4685880.6149277575236028No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3399120.4460663182056793No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2197850.2884237265728637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6296800.042.360271
AGCAGTG6589400.040.4065862
TCAACGC6894300.038.60083412
ATCAACG6901800.038.5934411
TGGTATC6888450.038.553267
GTGGTAT6897200.038.5191656
CAACGCA6919400.038.49083713
TATCAAC6947850.038.37597710
GTATCAA6936250.038.339159
GGTATCA6934600.038.329978
AGTGGTA6945200.038.278115
AACGCAG6990600.038.10679214
CAGTGGT6971400.038.105054
ACGCAGA7006050.037.9868315
AGAGTAC6954200.037.86116819
CGCAGAG7032250.037.8515116
GCAGAGT7045350.037.58128417
CAGAGTA7047250.037.51302718
GAGTACT6969450.037.50452420
GTACTTT6942650.037.4256322