FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n02_adt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n02_adt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40053088
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1946890.4860773781037807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCA13801600.043.8257061
GCATTCT3956200.043.8134881
TAACCAG13828600.043.787432
CCAGTTG13807750.043.722975
TTGTCCG8786300.043.7214285
GTACCCC13713750.043.7129911
TGTCCGA8793350.043.7024426
TACCCCC13660450.043.70241512
TCCGATT8781800.043.7012148
CTATTCA3532750.043.690643
TTGTACC13742500.043.684629
GTTGTAC13805700.043.684588
GTTGTCC8795250.043.680724
TGTACCC13740800.043.6713410
ACCAGTT13837900.043.6699374
AGTTGTC8845350.043.6695983
CCGATTC8750750.043.6688659
TATTCAG3541500.043.6591034
TCTATTC3531550.043.657192
GATTCCC8792350.043.64975411
Produced by FastQC (version 0.11.2)