FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_hto.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_hto.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24219733
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAA297900.020.0965375
CTGAAAC317400.019.918181
CCGGTAG239550.019.7879771
ACCGAGA304800.019.6760434
TACCGAG309250.019.5492083
CATATTC193400.019.4319361
TGGCGAA124250.019.4188529
CTTACCG344250.019.4050791
GAAGGTT336400.019.3791899
ACCTATA120500.019.2234942
GTCGGAG130100.019.2233077
AGTGAAC135700.019.219448
ACAGCCG152000.019.1895371
CGACACC116500.019.0701565
GAGAAGG344200.019.0678757
GTGGCGA126700.019.0598858
GCGACAC125500.019.0578784
TCATACG300950.018.9859878
CCGTGGA91700.018.9795281
GAGCGAC118800.018.9770832
Produced by FastQC (version 0.11.2)