FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_go.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_go.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43654398
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA174100.018.9558071
AGACTAG183600.018.7631938
GCATGCG301050.018.3310071
TGCCAAA156450.018.160451
ATGCGAG139800.018.1223243
TTGAACG131850.018.0754551
TTTATGC181600.018.0341281
TCACGTC170250.018.0138977
CCTCTGA147500.018.0090871
CATGCGA141450.017.897622
GACTAAC111400.017.86731
CTACCCA190050.017.8568131
ATTACAG166000.017.8489483
TCTGGAA127450.017.8094221
ACTGATG103200.017.7501341
ACTGAGT183050.017.7420851
GAATGAA122800.017.6968941
AAATGCC149600.017.6790541
GGGAATG123000.017.6766571
GAACCTA180950.017.6345371
Produced by FastQC (version 0.11.2)