FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cdna_d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cdna_d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78764942
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA241900.017.7813931
GACTAAC223000.017.6916581
CTGATAG307100.017.6796841
TGCCAAA306600.017.4961091
TATGCCC248600.017.479631
GAAATGA286450.017.4104371
ACTGAAC190100.017.3995631
CAATGGA262100.017.3856648
GGGCATC259300.017.276841
CTACCCA326050.017.251351
TTGGAAC236800.017.210671
ACTGATG179600.017.1476821
AGACGTT277150.017.115391
TGATTTC266300.017.1066931
TGACGGC189800.017.0950151
TTGAACG220400.017.0806431
GGGAATG217700.017.0705381
AAATGCC290450.016.9464471
CTCGAAA164900.016.9118711
GATCTAG289150.016.8991281
Produced by FastQC (version 0.11.2)