FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cdna_c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cdna_c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75449369
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC206050.017.9436321
CTGATAG298400.017.6985111
AGCTTGA238050.017.6450751
TGCCAAA300100.017.4757521
CAATGGA249950.017.4621358
TATGCCC241650.017.1996461
TTGGAAC222050.017.1852131
GGGAATG202600.017.1811961
ACTGAAC174100.017.1642071
GAAATGA272850.017.1461471
CTACCCA323700.017.0907421
GGGCATC247100.017.0795921
TGATTTC257450.017.0538981
TGACGGC172650.017.0406761
ACTGATG174750.017.0342481
AAATGCC274700.017.0230271
TTGAACG205800.017.0161191
TTTATGC254700.017.0112151
CTGAAAC197950.017.0064161
AGACGTT258750.016.9682181
Produced by FastQC (version 0.11.2)