FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cdna_b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cdna_b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124340384
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG481500.017.8179651
GACTAAC340450.017.780091
AGCTTGA394000.017.7734971
CAATGGA408900.017.4965888
TATGCCC403300.017.4860521
TGCCAAA484250.017.4261261
CTACCCA526900.017.3472061
ACTGAAC289150.017.3274171
GAAATGA457000.017.2746491
TGATTTC429700.017.1475071
GGGAATG336300.017.137471
AAATGCC453900.017.0987421
TGCTACC354100.017.026471
GGGCATC413550.017.0247141
TTGGAAC360000.017.0129341
TTTATGC411150.017.0091321
AGACGTT428700.016.9694461
CACCGAA243450.016.9233978
ACTGATG288300.016.853871
GACCTGG335250.016.8465081
Produced by FastQC (version 0.11.2)