FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cdna_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cdna_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76202122
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC208700.017.8410761
AGCTTGA238350.017.8118231
TATGCCC249350.017.5568081
TGCCAAA299750.017.4851191
CTGATAG303150.017.476111
ACTGAAC185500.017.4338381
CAATGGA250550.017.3616168
GAAATGA286250.017.2309741
CTACCCA313650.017.1790581
GGGCATC257350.017.1334781
AAATGCC285100.017.112591
TGACGGC176650.017.1061971
TGATTTC264300.017.04061
AGACGTT260250.017.0353831
TTTATGC254600.017.029761
GGGAATG205450.017.0292591
TTGAACG210950.016.9586931
TTGGAAC225100.016.9191881
GATGCTA169850.016.8820171
TGCTACC218750.016.819741
Produced by FastQC (version 0.11.2)