FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_adt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_adt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40053088
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAA543650.020.141345
CGTGTCC559300.020.0286210
ACCGAGA547950.020.0234724
CTGAAAC483300.019.9530411
TACCGAG552950.019.920363
GGCGACT334200.019.8662191
ACTGAAC289250.019.8487031
CCGGTAG303350.019.7778951
CTTACCG589900.019.7517911
GCGTGTC569250.019.7246239
GAGTTGG262850.019.7209019
GAAGGTT623300.019.5841529
AGTTGGC264700.019.53946110
TGTAAGC450200.019.4898684
ATGTAAG488250.019.4871353
AGAAGAA307500.019.4693788
AAGAAGC307100.019.4435510
GAGCCTC185700.019.432733
GACTAAC203950.019.4157221
CAATAAG317350.019.3845889
Produced by FastQC (version 0.11.2)