FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37861375
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3525320.9311125124219604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN544620.14384580591698004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT30600.045.3344965
GGGCGCT13900.044.9048083
CCGCTAG19550.043.542625
AGCGCTA21400.042.5955434
CGCTAGA33800.041.541496
GCGCTAG18450.040.8222665
CCCGCTA21400.040.6295934
CAGCGCT16500.039.5290763
GCGCTAA24250.037.1258475
GCGCTCA17850.036.6993525
CGCTAGT22450.035.8728526
CCGCTAT40400.035.638185
GTCCTAT50600.035.03125
CGCTATA44800.034.624236
GAGCGCT15050.034.2506263
TAGCGCT13300.033.7478453
CACTGTA51600.033.0439228
GCGCTCC11400.031.9582885
AGCGCTC16900.031.9476244
GTGCGCT11200.031.9352113
Produced by FastQC (version 0.11.5)