FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_SPL9_2019poolG_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_SPL9_2019poolG_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11643040
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340890.292784358724182No Hit
CCGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG173170.14873263340158582Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT323000.063.781475
GGTCCTA148400.063.397784
GCGAGTA113650.062.302454
TGTCCTA307100.061.3854144
CGGGAGT70750.060.801123
GGGAGTA148850.060.7371254
GGAGTAT196600.060.5836225
CGAGTAT206300.060.4835245
CGGTCCT74600.060.337633
GATCCTA247000.060.2808274
TCCTATA316150.059.8589136
ATCCTAT309250.059.8379065
TAGTCCT223650.059.6112483
AGGTCCT148450.059.5107353
TCCTATG187950.059.422396
CGGAGTA123950.059.412514
GTCCTAA231250.059.3460585
CGCGAGT55600.059.301033
AGTCCTA268300.059.08274
GGCGAGT56800.059.034153
Produced by FastQC (version 0.11.5)