FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_Col_UB_g3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_Col_UB_g3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723520
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC500.00720908327.9996412
GGCCAGG500.00720908327.999646
CGGACTG1207.402741E-826.2496646
GGACTGT1202.1673368E-623.3330357
AGGAACG900.005256459619.4562971
TCGGACT900.005273294619.445545
GACTGTA1859.027957E-515.134948
ACTGTAG1901.1331153E-414.7366529
CTACAGT1450.004738055614.48257324
CTGTAGA2253.0303952E-513.99982110
CGTCGTG1750.001017404313.9998251
GTAGAAG3750.0034044528.39989312
Produced by FastQC (version 0.11.5)