FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_Col_UB_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_Col_UB_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53437
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG150.002213273770.010
AGTCCGA150.002213273770.028
TAGGTTC150.002213273770.023
ATCGGGT150.002213273770.08
CTGTAGA550.063.63636810
ACTGTAG601.8189894E-1258.3333329
CGGACTG601.8189894E-1258.3333326
GACTGTA653.6379788E-1253.8461538
GATCGGG200.00691597252.57
AGGAGGG200.00691597252.521
ACGGACT407.3495903E-752.55
ACCAATA200.00691597252.567
ACGGTCA200.00691597252.515
GGACTGT707.2759576E-1250.07
GTAGAAG802.7284841E-1143.7512
CTACAGT503.418736E-642.00000424
CAGTCCG503.418736E-642.00000427
TACAGTC503.418736E-642.00000425
ACAGTCC556.5800887E-638.1818226
GAAGATC708.4952444E-735.015
Produced by FastQC (version 0.11.5)