FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_Col_UB_g1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_Col_UB_g1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886587
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17990.20291296849604157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4450.060.5613566
ACTGTAG13300.056.315389
TCCACTG11450.056.2441376
ACTAGTG6550.055.037779
CCACTGT10000.054.94967
GGACTGT4700.054.361317
CACTGTA9800.053.571048
CCACTAG7450.053.5566567
CTGTAGA13900.052.62551510
CTAGTGG7000.050.9996310
CACTAGT7350.049.0472648
GACTGTA5350.046.448268
ATCCACT10050.045.9698145
CCACTCT6100.045.3275387
GTCCACT5750.045.043155
TCCACTA10050.044.576796
CCGGACT1900.044.210215
GTAGAAG16650.043.513212
TCCACTC7000.043.4996836
CTCCACT7350.043.333025
Produced by FastQC (version 0.11.5)