FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_Col_B_g2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_Col_B_g2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27199
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280.10294496121180925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAA150.002201516670.05
TCCTTAT207.7902405E-570.06
CCTAATA150.002201516670.07
GGTCCTA150.002201516670.04
TATCCTA252.7669485E-670.04
GTCCTAA252.7669485E-670.05
GAGTAAA207.7902405E-570.06
ATATCCT150.002201516670.03
CTATCCT150.002201516670.03
CCTAATC252.3495559E-455.9999967
AGTCCTA252.3495559E-455.9999964
ATCCTCT200.00687951752.55
GGTTGAT200.00687951752.53
TCCTATA200.00687951752.56
CACGTCA200.00687951752.551
GAAGTCC200.00687951752.52
CCTTATT200.00687951752.57
TCCTAAT505.9062586E-848.9999966
CTAATAA305.7780097E-446.6666648
AAGTCCT305.7780097E-446.6666643
Produced by FastQC (version 0.11.5)