FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_2019poolF_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_2019poolF_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519456
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144170.5722267029072943No Hit
CCCCTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG120110.4767298972476598Illumina Single End PCR Primer 1 (96% over 33bp)
CCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG110190.43735631818932347Illumina Single End PCR Primer 1 (96% over 33bp)
CCACCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG56020.22234958657741988Illumina Single End PCR Primer 1 (96% over 33bp)
CCAATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG32340.1283610430188104Illumina Single End PCR Primer 1 (96% over 33bp)
CCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG31710.12586050321974268Illumina Single End PCR Primer 1 (96% over 33bp)
CCACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTG26260.10422884940241067Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT29950.067.4295043
GCGAGTA55800.067.24064
CGGGAGT35600.066.854393
GGGAGTC37050.066.7885744
GGAGTAT104100.066.772795
CTGAGTA75200.066.6028444
GGGAGTA68500.066.5264
CCCGAGT38400.066.3546143
ACGCGAG20000.066.326772
CGAGTAT104900.066.296935
CCGAGTC35550.066.259244
GGCGAGT26250.066.000453
GGGGAGT36350.065.9564363
GAGTATT138950.065.9437266
CCGAGTA66900.065.867414
CGGAGTA46400.065.7008744
CGAGTCT61950.065.5936665
AGGAGTA69800.065.537694
GCACTCG24250.065.380598
CGTATCA162150.065.3583868
Produced by FastQC (version 0.11.5)