FastQCFastQC Report
Wed 6 Mar 2019
HGWH2AFXY_n02_ColTGA5_2019poolE_B_g.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWH2AFXY_n02_ColTGA5_2019poolE_B_g.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773462
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116560.14994606006950315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT310000.067.50665
GGTCCTA195750.066.9983444
AGGTCCT188950.066.262133
AGTCCTA312600.066.2289664
TGTCCTA259300.066.1012654
GTCCTAA268350.066.036755
CGGTCCT79750.065.6593
CGTCCTA93350.065.540434
GTCCTAC116250.065.485585
GAGTCCT216650.065.4157643
GTCCTAG165550.065.32945
GATCCTA365200.065.325234
TCCTATA393150.065.058326
ATCCTAT455900.064.604675
TCCTATG246750.064.424726
ATGTCCT228900.064.361553
TAGTCCT230600.064.357613
GTCCTGT115450.064.241615
TCCTAGA295750.064.212216
GGTCCTC112250.064.202544
Produced by FastQC (version 0.11.5)